Coding

Part:BBa_K4345016:Design

Designed by: Paula Ventura   Group: iGEM22_KU_Leuven   (2022-09-30)


ccdB with STOP codon control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A STOP codon control was added to this sequence.


Source

E. coli genome

References

Bahassi, E. M., O’Dea, M. H., Allali, N., Messens, J., Gellert, M., & Couturier, M. (1999, April). Interactions of CcdB with DNA Gyrase. Journal of Biological Chemistry, 274(16), 10936–10944. https://doi.org/10.1074/jbc.274.16.10936